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Go to the iLab’s website, https://uncch.ilab.agilent.com/service_center/show_external/4429

Click “Sign in” at the top right corner of the page, sign in with your UNC credentials, then select the “Request Services” tab on the top right menu.

  • Login using your UNC affiliated email address and password
  • Click on the core facilities link in the left hand menu
  • Select “CGIBD-Histology” from the list of cores
  • On the core’s page, click the ‘about our core’ tab to learn more about the available services.

  • To request services, click the ‘request services’ tab.
  • Select ”Initiate request” from the Sample Request row to submit a work order

  • If you belong to multiple labs, please select the lab from the drop down menu that you wish to make the order for. If you are only apart of one lab, then the form will automatically forward you to the request form itself.
  • Now you are ready to purchase

Example 1: Customer needs 10 samples processed and embedded only

  • If you need processing and embedding only, then scroll down to processing and click “yes”. Place the # of cassettes you are submitting in the box and then select “Add selected Services”. If you do not bring yours samples in labeled cassettes in 70% ethanol, you will need to add the grossing charge per sample.

  • Scroll down to embedding, click that “yes” embedding is required.  Place the # of cassettes you are submitting in the box and select “Add selected services” for the embedding row.

  • Double check and click on the “Submit request to researcher” button on the bottom right screen. Please print out your request sheet after submitting.  Bring the Sheet and Samples to the CGIBD Histology Core located at 030 Glaxo Research Building. You may place them in the drop off red bin on the blue cart.

Example 2: Customer needs 10 cassettes processed and embedded, 3 slides each block 2 sections per slide, the first slide stained H&E and 2 unstained slides per block for IHC.

  • If you need processing and embedding, then scroll down to processing and click “yes”.  Place the # of cassettes you are submitting in the box and select “Add selected Services”.

  • Scroll down to embedding and click on “yes”.  Place the # of cassettes you are submitting in the box and select “Add selected Services”.

  • Next you will select Sectioning by clicking on the “yes” button. Now fill out the required parts of the form denoted by stars to specify how you would like your tissue to be sectioned; then tally the total number of sections for all blocks to be sectioned.  Finally, select “Add selected Services”.

  • Next select Staining: Although the customer requested 30 slides, you only want 10 stained H&E. Next you will select Staining by clicking on the “yes” button. Then fill out the number of slides to be stained and select “Add selected Services”.

  • In addition, customer will need a 100 place slide box; Select Material Costs by clicking on the “yes” option. Then specify how many of a slide box that you will need and select “Add selected service”.

  • Double check and click on the “Submit request to researcher” button on the bottom right screen. Please print out your request sheet after submitting.  Bring the Sheet and Samples to the CGIBD Histology Core located at 030 Glaxo Research Building. You may place them in the drop off red bin on the blue cart.

Example 3: Customer has 5 previous embedded blocks  and wants 10 slides per block, 1 section per slide, each first slide stained H&E.

  • First, you will select Sectioning by clicking on the “yes” button. Please note, that you do not need to request processing or embedding services because the blocks are already embedded. Now fill out the required parts of the form denoted by stars to specify how you would like your tissue to be sectioned; then tally the total number of sections for all blocks to be sectioned.  Select “Add selected Services”.

  • Next select Staining: Although the customer requested 50 slides, you only want 10 stained H&E. Next you will select Staining by clicking on the “yes” button. Then fill out the number of slides to be stained and select “Add selected Services”.

  • In addition, customer will need a 100 place slide box; Select Material Costs by clicking on the “yes” option. Then specify how many of a slide box that you will need and select “Add selected service”.

  • Double check and click on the “Submit request to researcher” button on the bottom right screen. Please print out your request sheet after submitting.  Bring the Sheet and Samples to the CGIBD Histology Core located at 030 Glaxo Research Building. You may place them in the drop off red bin on the blue cart.

Example 4: Customer has 30 Trichrome stained slides and wants all 30 of the slides to be scanned.

  • If you need slides to be scanned, then scroll down to scanning and click “yes”.  Place the # of slides that you are submitting in the box and select “Add selected Services”.

  • Double check and click on the “Submit request to researcher” button on the bottom right screen. Please print out your request sheet after submitting.  Bring the Sheet and Samples to the CGIBD Histology Core located at 030 Glaxo Research Building. You may place them in the drop off red bin on the blue cart.